Journal: Cellular and Molecular Life Sciences

Article Title: Decreased expression of the NLRP6 inflammasome is associated with increased intestinal permeability and inflammation in obesity with type 2 diabetes

doi: 10.1007/s00018-024-05124-3

Figure Lengend Snippet: Gene expression levels of the inflammasome components ( NLRP3 and NLRP6 ), their main effectors ( IL1B and IL18 ) and the intestinal integrity genes ( MUC2 and TJP1 ) in human enterocytes CCL-241 cells treated with different concentrations of A tumor necrosis factor (TNF)-α, B interleukin (IL)-1β, C adipocyte conditioned media (ACM) and D taurine and histamine during 24 h ( n = 6 per group). E Effect of lipopolysaccharide (LPS), parthenolide (PTL) and LPS for 3 h followed by PTL for another 4 h in the regulation of inflammasome components and inflammation and intestinal integrity genes in CCL-241 cells. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Dunnett’s tests. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs unstimulated cells. NLRP nucleotide-binding oligomerization domain, leucine rich repeat and pyrin, IL interleukin, MUC2 mucin 2, TJP1 tight junction protein-1

Article Snippet: The BCM was centrifuged and diluted at 40% in RPMI-1640 (Merck) (for the treatment of CCL-241 cells) and in DMEM/F-12 (for adipocyte treatment).

Techniques: Expressing, Binding Assay

Journal: Cellular and Molecular Life Sciences

Article Title: Decreased expression of the NLRP6 inflammasome is associated with increased intestinal permeability and inflammation in obesity with type 2 diabetes

doi: 10.1007/s00018-024-05124-3

Figure Lengend Snippet: Effect of blocking NLRP6 expression in A the gene expression levels of interleukin-18 ( IL18 ), mucin 2 ( MUC2 ), tight junction protein 1 ( TJP1 ), adiponectin ( ADIPOQ ) and interleulin-1β ( IL1B ) and B the release of mucin-2 into the culture media. CCL-241 cells were transfected with or without 200 pmol/L NLRP6 siRNA/2 × 10 5 cells/well for 24 h. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by unpaired Student’s t -test. * P < 0.05 and ** P < 0.01

Article Snippet: The BCM was centrifuged and diluted at 40% in RPMI-1640 (Merck) (for the treatment of CCL-241 cells) and in DMEM/F-12 (for adipocyte treatment).

Techniques: Blocking Assay, Expressing, Transfection

Journal: Cellular and Molecular Life Sciences

Article Title: Decreased expression of the NLRP6 inflammasome is associated with increased intestinal permeability and inflammation in obesity with type 2 diabetes

doi: 10.1007/s00018-024-05124-3

Figure Lengend Snippet: Gene expression levels of NLRP3 , NLRP6 and MUC2 in A CCL-241 cells and C visceral adipocytes after the incubation with heat-inactivated Akkermansia muciniphila and with the bacteria-conditioned medium (BCM) (40%) for 24 h. Mucin-2 concentrations in the culture media of B CCL-241 cells and D visceral adipocytes incubated in the presence of heat-inactivated A. muciniphila and with the bacteria-conditioned medium (BCM) (40%) for 24 h. E Gene expression levels of CLDN1 and OCLN in CCL-241 cells after the incubation with heat-inactivated Akkermansia muciniphila and with the bacteria-conditioned medium (BCM) (40%) for 24 h. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. * P < 0.05, ** P < 0.01 and *** P < 0.001. BHI brain heart infusion, CLDN1 claudin 1, MUC2 mucin 2, NLRP nucleotide-binding oligomerization domain, leucine rich repeat and pyrin, OCLN occludin

Article Snippet: The BCM was centrifuged and diluted at 40% in RPMI-1640 (Merck) (for the treatment of CCL-241 cells) and in DMEM/F-12 (for adipocyte treatment).

Techniques: Expressing, Incubation, Bacteria, Binding Assay